Associations between anther-culture response and molecular markers on chromosomes 2H, 3H and 4H of barley ( Hordeum vulgare L.). Identification of a rice gene ( Bph1 ) conferring resistance to brown planthopper ( Nilaparvata lugens Stål) using STS markers. A RAPD marker for the gene conferring resistance to Indian biotype of BPH. Jeon Y H, Ahn S N, Choi H C, Hahn T R, Moon H P. Manila, Philippines: International Rice Research Institute: 29. RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown planthopper resistance in a doubled haploid rice population. Huang N, Arnold P, Mew T, Magpantay G, McCouch S, Guiderdoni E, Xu J, Subudhi P, Angeles R, Khush G S. From secondary to major pest status: The case of insectcticide-induced rice brown planthopper, Nilaparvata lugens resurgence. Leafhopper Vectors and Planthopper Disease Agents. Control of leafhopper and planthopper vectors of rice viruses. Genetics of resistance to ascochyta blight ( Ascochyta lentis ) of lentil and the identification of closely linked RAPD markers. Key DNA markers for predicting heterosis in F 1 hybrids of japonica rice. Cho Y I, Park C W, Kwon S W, Chin J H, Ji H S, Park K J, McCouch S, Koh H J. Prabhakara R AO, Scientist, CTRI, Rajahmundry (AP) for improving the manuscript and reviewers for suggesting the better representation of this article. We sincerely acknowledge the Department of Biotechnology, Government of India, for providing financial assistance and Dr. In summary, the co-dominant RAPD markers OPC7 697 and OPAG14 680, which are associated with coupling phase to the resistant allele of brown planthopper resistance, could be used in marker-assisted selection in plant breeding program as well as in identifying genes at any stage of the crop growth period to save time and cost of traditional breeding programs. Poulson et al (1995) showed that the bulked segregant analysis is the straight forward method to cope with the low levels of phenotypic misclassification, especially with bulks constructed with sufficiency number of individuals. Thus, bulked segregant analysis with RAPD markers provides the marker- assisted selection in the F 2 population of IR50 and Ptb33. We have shown a clear cut polymorphism between susceptible individuals and resistant ones in respect of BPH resistance in the bulked segregant analysis. Several such co-dominant RAPD markers have been successfully developed for marker-assisted selection in several crops (Mondal et al, 2007). Thereby, co-dominant markers can easily distinguish between recombinant homozygotes and recombinant heterozygotes (Williams et al, 1990 Mohan et al, 1997 Semagn et al, 2006). For linkage analysis, co-dominant markers provide the maximum linkage information per individual in the mapping population under the study. Here, the two pools or bulks contrasting for BPH resistance were distinguished by two co-dominant markers. Thus, OPC7 and OPAG14 are considered to be co-dominant primers, and such type of co-dominant markers would provide more information than either dominant or recessive type of markers in marker-assisted selection. Of which, OPC7 697 (697 bp) and OPAG14 680 (680 bp) were associated in coupling phase to the resistant allele in resistant parent, resistant bulk and all ten resistant F 2 seedlings, whereas OPC7 846 (846 bp) and OPAG14 650 (650 bp) were linked in repulsion phase. 3 showed the polymorphic DNA fragments of OPC7 and OPAG14, respectively. In the bulked segregant analysis, the minimum size of either resistant or susceptible bulk depends on the frequency of linked loci that is detected as polymorphic DNA fragments between the bulked samples. The alternative to this step is the bulked segregant analysis, proposed by Michelmore et al (1991). This is the most time consuming step for DNA marker development. The next step is the screening of the entire population either by probes or with primers. Such high number of amplified loci is essential for tagging of resistance genes (Welsh and McClelland, 1990). The data from Table 1 showed high number of amplified products per primer.
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1/14/2024 0 Comments Base of an isosceles triangle![]() We get the cos of 34 degrees is equal to the length of □□ divided by nine. Now, we can just substitute these values into this formula. We recall if □ is an angle in a right triangle, then the cos of □ is equal to the length of the side adjacent to angle □ divided by the length of the hypotenuse. Therefore, we need to use the cosine ratio. So, we know the length of the hypotenuse and we want to determine the length of the adjacent side. And we only know the length of the hypotenuse in this right triangle. We want to find the length of the line segment □□, and we’re doing this by finding the length of the line segment □□, which is our adjacent side. We’re now ready to apply our acronym to determine which of the trigonometric ratios we need to use. Finally, we can notice that side □□ is adjacent to this angle, so we label side □□ as the adjacent side. Next, we can see that the side □□ is opposite the angle 34 degrees, so we’ll label this as the opposite side. We can see that this is side □□ we’ll label this as the hypotenuse. We’re going to use triangle □□□.įirst, we’re going to label the hypotenuse as the longest side of the triangle, which is the one opposite the right angle. But first, we need to label the sides of our right triangle based on their position relative to the angle of 34 degrees. This will help us determine which of the three trigonometric ratios we need to use to determine the side length. To do this, we start by recalling the acronym SOH CAH TOA. We now want to apply right triangle trigonometry to determine the length of these two sides. However, we could’ve also showed this by using trigonometry. And in particular, this tells us the length of □□ is equal to the length of □□. Therefore, by the angle-angle-side congruence criterion, these two triangles are congruent. To do this, we first note that the triangles share two angles and the side length in common. We can notice that triangles □□□ and □□□ are congruent. However, there’s one extra simplification we can use. Adding these together would then give us the length of the line segment □□.Īnd this would work and give us the correct answer. Therefore, by using right triangle trigonometry, we could determine the length of line segment □□ and the length of line segment □□. And we recall if we know one of the non-right angles of a right triangle and one of the side lengths, we can use right triangle trigonometry to determine the other side length in the right triangle. However, the easiest way is to notice that □□□ and □□□ are both right triangles. There’s many different ways we can go about this. We need to give our answer to one decimal place. We need to use all of this information to determine the length of line segment □□. ![]() ![]() Therefore, the measure of angle □ is 34 degrees. And since the sides □□ and □□ have equal length, the measure of angle □ must be equal to the measure of angle □. But remember, this is an isosceles triangle. We’re also told that the measure of angle □ is 34 degrees. □□□ and □□□ are both right triangles with right angles at □. And we’re told that □□ is perpendicular to □□. In the diagram, we can also see that we have a line from □ to □, where □ is a point on the base of the triangle. First, we’re told that sides □□ and □□ have equal length. In this question, we’re given some information about an isosceles triangle □□□. Find the length of the line segment □□, giving the answer to one decimal place.
1/14/2024 0 Comments Call of duty black ops 3 pc![]() Because that is reportedly the only option that works. Therefore, in this step, we will be disabling the Antivirus temporarily but only for 5 minutes. Many AVG users have been facing this issue with Black Ops 3 and the game stops working for them sometime after launching. Also, try running the computer in clean boot and then check to see if the issue still persists in that state.Close the Process Explorer and check to see if the game runs fine.Turn off all Antiviruses temporarily for at least 10 minutes. ![]() Press Ctrl + Shift + Del and try closing some background processes from there.In the NVIDIA Control Panel, go to Program Settings > Black Ops > Manager 3D Settings and then change the Power Management Mode to Prefer maximum performance.There seems to be some incompatibility betweens Black Ops II and FRAPS, so you can give that a try. Notice you’ll be exposing your PC to threats if you do this. You can do this by typing firewall in the Start Menu and pressing Enter, clicking Turn Windows Firewall on or off and then turning the firewall off. This may create additional files in the game which should make the game work normally. If by now none of these fixes have worked, then try any of these additional fixes as they might work for you. Try running the game now and it should hopefully run this time.Finally, right-click on the application icon and select New > Desktop Shortcut.Check the “ Disable display scaling on high DPI Settings” box.Check “ Reduced color mode” and set it to 16-bit.Back to the Settings tab, apply the following settings and click on OK.check Run this Program in Compatibility Mode Click on the Compatibility tab, check the “ Run this program in compatibility mode for” box and select Windows 7 from the dropdown list.In that folder, right-click on exe and click on Properties.Paste the following path in the address bar and hit the Enter key: C:\Program File (x86)\Steam\steamapps\common\Call of Duty Black Ops. Press the Windows + E keys to open Windows Explorer.Here’s what to do to get the game running in this case. If you’re using a 64-bit OS, note that Black Ops II is a 32-bit game and may have issues running it. 1/14/2024 0 Comments Avast crashplanWould highly recommend you look into setting something like this up as it will run way more natively on the NAS, especially in conjunction with way more full-featured backup apps like duplicacy or borg. The prevailing expectation with cloud storage providers appears to be that you use B2 or some such to store your backups (Google Workspace may be a fixed price option too). All of these together led me to close out my CP sub after 10+ years. On top of which - they have been promising an update to that Java app ever since they upped their prices, which does not look like it’s happening. This is completely unacceptable to me for a backup. Also a number of recent threads with horror stories of restoring from CP (search for them): lost/corrupted data, archives inexplicably and irrevocably unavailable in a disaster recovery scenario etc. One thing to keep in mind is that CP is clearly cracking down on this use-case and keeps closing off ways in which people have been running their app on NAS boxes. The VM approach would be a feasible solution on the face of it, though the docker image has been kept well up to date in my past experience. Mount the desired NAS shares on the virtual machine (this will make the shares available to the virtual machine's file system).Ĭonfigure CrashPlan to create offsite backups of the mounted shares.Ĭould this be a feasible solution to the problem described above? Install CrashPlan on the virtual machine. Install a Windows 10 or Ubuntu virtual machine on the NAS using the Virtual Machine Manager.Īllocate a sufficient amount of RAM to the virtual machine (CrashPlan is based on Java and eats up a lot of RAM). Should we circumvent this problem by following the steps below? IMHO, it looks like a makeshift solution that may stop working if the developer decides to abandon the project. ![]() The following Docker solution, which seems to be maintained by only one person, appears to be the only one that works currently: The problem, however, is that CrashPlan is nowhere to be found in the Package Center on DSM v6.2.3 even if you set the trust level to "any publisher".Ī compatible version of CrashPlan used to be available at, which is a community source, but the package no longer works properly. Currently, we use CrashPlan as our online backup and file hosting service. We have a Synology NAS with a whopping 64 GB of RAM, and we'd like to dedicate the NAS to handling all offsite backups. See External resources below for links to instructions for commonly used antivirus applications, as well as Windows and Mac firewalls.I've already asked this elsewhere, but for good measure, I'm posting it here too. If you are still unable to connect, repeat Step 1: Manually attempt to reconnect. ![]() If possible, create an exception or rule to allow traffic addressed to *(using a wildcard in the exception allows traffic to any our servers)Īfter adding antivirus software or firewall exceptions, open the CrashPlan app to check your connection status.If possible, create an exception or rule to allow outgoing communication on TCP port 443.Create an exception or rule for CrashPlan for Small Business.Consult the application's documentation to: ![]() If CrashPlan for Small Business connects to the destination with the antivirus or firewall applications disabled, then there is a conflict between CrashPlan for Small Business and the application or firewall.
1/13/2024 0 Comments The grommet window cleaner![]() ![]() My friends at the time from the vagboard (does anyone still know that?), were the pioneers in “rear wiper removal”. So I had the idea of making something that is easy to install and looks good: Our first product in 2007 was a milled aluminum wiper hole insert that I primed by hand in the garage and then painted and sold. So remove… Seal the wiper water line … and what happens to the hole in the window? When I was looking for ways to close the rear wiper hole, I only found a chunky plastic nub in the hardware store. I was satisfied, but it always bothered me that the asymmetrically positioned rear window wiper disturbed the rear view. Everything is as high quality as possible but reduced to the essentials. We invented the wiper hole plugs for the rear window in 2007: I was in the tuning scene for years and with my own “JTI 2006 project” I wanted to tick off all vehicle refinement checkboxes: FFF (chassis, rims, finished) + Alcantara interior, beads removed/cleaned and so on. Small (glass) plug, big effect – remove the rear wiper and replace it with a suitable glass insert – clean rear view of the car with minimal tuning effort! Glass flush wiper delete kits from – the ORIGINAL! ![]() If you are still dissatisfied, please contact us, we will exchange unsuitable glass covers free of charge (if they are not damaged) – and if you dropped the stopper or accidentally damaged it when you tried it, we will find a fair solution and send it to you inexpensively Replacement – so don’t worry … write to us at and we will be there for you as soon as possible.Īrteon (3H), Bora (1y), Caddy (2K), Caddy (2K/2KN), Caddy (2K/2KN) Facelift, Caddy (2K/2KN) facelift, Caddy (2KN), Crafter (2EKE1), Crafter (2FJE1), Crafter (SY* / SZ*), Crafter (symwe), Crafters (2EC1), Fox (5Z), Golf IV (1Y), Golf V (1K), Golf V (1KM), Golf V (1KP), Golf VI (1K), Golf VI (1KM), Golf VI (1KP), Golf VII (1K), Golf VII (1K) Facelift, Golf VII (1KM) Facelift, Golf VII (AU), Golf VII (AU) facelift, Golf VII (AUV), Golf VII (AUV) facelift, Golf VIII (CD), Golf VIII (CDV), Gulf VII (1KM), ID.3 (E1), Lupo (6X/6E/6ES), Passat (3B), Passat (3BG), Passat (3C), Passat (3C) facelift, Polo (6N), Polo (6N) 6N2 / Facelift, Polo (6R), Polo (6R) facelift, polo (9N), Polo (9N) 9N3 / Facelift, polo (9N2), Polo (AW), Sharan (7M), Sharan (7M) Facelift I, Sharan (7M) Facelift II, Sharan (7N), Sharan (7N) Facelift, T Cross (C1), T Roc (A1), T5 (7HC), T5 (7HC) Facelift, T5 (7HC/7HCA), T5 (7HK), T5 (7HK/7HKX0), T5 (7HM), T5 (7HM) Facelift, T5 (7HM/7HMA), T5 (7HM/7HMA) Facelift, T5 (7J0) Facelift, T6 (7HC), T6 (7HMA), Tiguan (5N), Tiguan (5N) facelift, Touareg (7L), Touareg (7L) facelift, Touareg (7P/7PH), Touareg (7P/7PH) Facelift, Touareg (CR), Touran (1T), Touran (1T) Facelift, Touran (1T) Facelift I, Touran (1T) Facelift II ![]() That is why we polish the visible edge of the glass insert by hand and our team subjects every stopper to a precise quality check before it is sent to you. Oh and important: the back of our stopper is coated in black and the front is covered with a transparent protective film so that nothing is scratched when it is inserted into the rear window – so please do not be surprised about the dull / milky appearance of the stopper and simply remove the protective film after inserting it into the window pull off.Ī perfectly clean OEM look is our top priority. You can find a repair guide for removing and installing the rear window wiper in our help desk. The washer fluid nozzle is sealed and, in newer vehicles, the CAN bus is coded. Instead of replacing / repairing the defective wiper arm or wiper motor at the rear, with our sealing plugs we offer a simple and inexpensive replacement part that everyone can install themselves. Some people want to know how to repair their defective rear wiper and find out how expensive a repair is. If you remove the (possibly defective )) rear window wiper from your car, a small hole remains in the rear window, which you can close flush with our glass stopper. Our wiper hole covers are milled from a piece of float glass and are individually tailored to your vehicle. We are the inventors of the rear wiper cover made of glass. 1/13/2024 0 Comments Subway surfers apk hack 4 shared![]() Zombie Highway 2 is one of the best alternative of Subway surfer, defend yourself from various Zombie simulation made in-game while you defend yourself from vehicles. Graphics isn’t the matter, but it indeed keeps you on track. Crossy road it a simulation of Subway surfer gameplay where you have to cross through street of traffic. It is time to experience the vibes of city and chaos that we see in our daily life. So what are you waiting for? It is time to dive in the speed now. The game revolves around similar game play to Subway surfer but with a unique crush of Sonic. Speed! 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If you have not heard about Subway Surfers by now, then you must have been living under a non-gaming rock. 1/13/2024 0 Comments Marty schwartz![]() “He was looking to generate money, but the products he came up with weren’t viable. “He called me to discuss products he could sell on QVC,” recalls his longtime secretary, Alice Marchak. Robin Williams attended all 10 days, at one point doing a 30-minute improv routine about haggling with a used-car salesman. He always had a reason.”) While a jazz musician played Brando’s favorite tunes on a rented piano, Philippe Petit, the French tightrope walker who had crossed the Twin Towers, did stunts on a high-wire. (“The girls were shaking, like, ‘What the f- am I doing here?’ ” recalls Olmos. He had students strip naked in front of the entire class. Another time, Brando plucked a homeless man from a dumpster and brought him in for acting lessons. ![]() It also featured some of the strangest, and some would say finest, performances of Brando’s later years.ĭuring one of the sessions, a troupe of little people and a team of Samoan wrestlers - Brando somehow had wrangled all of them to the warehouse on the same day - did improvisation exercises together on the stage. The event is little recalled today - and even back then it slipped mostly under the radar - but those daylong classes, where movie stars mingled with midgets, Madonna‘s ex-boyfriend nearly caused a riot and an Osama bin Laden lookalike almost gave Jon Voight a coronary, was a never-to-be-repeated moment of Hollywood letting its freak flag fly. “I am furious! Furious!” Brando told the group in a matronly English accent, launching into an improvised monologue that ended, 10 minutes later, with the actor turning around, lifting his gown and mooning the crowd.Īnd that, it turned out, would be one of the more decorous moments of “Lying for a Living,” the wild 10-day symposium - as much a 1960s– style “happening” as it was an acting course - that Brando organized and led in November 2001, less than three years before his death. Waving a single rose in one hand, he sashayed through the warehouse, plunked his 300-pound frame onto a thronelike chair on a makeshift stage and began fussily applying lipstick. 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