Associations between anther-culture response and molecular markers on chromosomes 2H, 3H and 4H of barley ( Hordeum vulgare L.). Identification of a rice gene ( Bph1 ) conferring resistance to brown planthopper ( Nilaparvata lugens Stål) using STS markers. A RAPD marker for the gene conferring resistance to Indian biotype of BPH. Jeon Y H, Ahn S N, Choi H C, Hahn T R, Moon H P. Manila, Philippines: International Rice Research Institute: 29. RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown planthopper resistance in a doubled haploid rice population. Huang N, Arnold P, Mew T, Magpantay G, McCouch S, Guiderdoni E, Xu J, Subudhi P, Angeles R, Khush G S. From secondary to major pest status: The case of insectcticide-induced rice brown planthopper, Nilaparvata lugens resurgence. Leafhopper Vectors and Planthopper Disease Agents. Control of leafhopper and planthopper vectors of rice viruses. Genetics of resistance to ascochyta blight ( Ascochyta lentis ) of lentil and the identification of closely linked RAPD markers. Key DNA markers for predicting heterosis in F 1 hybrids of japonica rice. Cho Y I, Park C W, Kwon S W, Chin J H, Ji H S, Park K J, McCouch S, Koh H J. Prabhakara R AO, Scientist, CTRI, Rajahmundry (AP) for improving the manuscript and reviewers for suggesting the better representation of this article. We sincerely acknowledge the Department of Biotechnology, Government of India, for providing financial assistance and Dr. In summary, the co-dominant RAPD markers OPC7 697 and OPAG14 680, which are associated with coupling phase to the resistant allele of brown planthopper resistance, could be used in marker-assisted selection in plant breeding program as well as in identifying genes at any stage of the crop growth period to save time and cost of traditional breeding programs. Poulson et al (1995) showed that the bulked segregant analysis is the straight forward method to cope with the low levels of phenotypic misclassification, especially with bulks constructed with sufficiency number of individuals. Thus, bulked segregant analysis with RAPD markers provides the marker- assisted selection in the F 2 population of IR50 and Ptb33. We have shown a clear cut polymorphism between susceptible individuals and resistant ones in respect of BPH resistance in the bulked segregant analysis. Several such co-dominant RAPD markers have been successfully developed for marker-assisted selection in several crops (Mondal et al, 2007). Thereby, co-dominant markers can easily distinguish between recombinant homozygotes and recombinant heterozygotes (Williams et al, 1990 Mohan et al, 1997 Semagn et al, 2006). For linkage analysis, co-dominant markers provide the maximum linkage information per individual in the mapping population under the study. Here, the two pools or bulks contrasting for BPH resistance were distinguished by two co-dominant markers. Thus, OPC7 and OPAG14 are considered to be co-dominant primers, and such type of co-dominant markers would provide more information than either dominant or recessive type of markers in marker-assisted selection. Of which, OPC7 697 (697 bp) and OPAG14 680 (680 bp) were associated in coupling phase to the resistant allele in resistant parent, resistant bulk and all ten resistant F 2 seedlings, whereas OPC7 846 (846 bp) and OPAG14 650 (650 bp) were linked in repulsion phase. 3 showed the polymorphic DNA fragments of OPC7 and OPAG14, respectively. In the bulked segregant analysis, the minimum size of either resistant or susceptible bulk depends on the frequency of linked loci that is detected as polymorphic DNA fragments between the bulked samples. The alternative to this step is the bulked segregant analysis, proposed by Michelmore et al (1991). This is the most time consuming step for DNA marker development. The next step is the screening of the entire population either by probes or with primers. Such high number of amplified loci is essential for tagging of resistance genes (Welsh and McClelland, 1990). The data from Table 1 showed high number of amplified products per primer.
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